600-401-677S

antibody from Rockland Immunochemicals, Inc.

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Provider product page for 600-401-677S

Western blot

Antibody data

Antibody Data
Antigen structure
References [27]
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Validations
Western blot [1]

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Product number: 600-401-677S - Provider product page
Provider: Rockland Immunochemicals, Inc.
Proper citation: Rockland Cat#600-401-677S, RRID:AB_11183440
Product name: Anti-Aurora B pT232 (RABBIT) Antibody - 600-401-677S
Antibody type: Polyclonal
Vial size: 25 µl

AURKB protein structure - 600-401-677S shown in red.

Submitted references SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation.

Zhang M, Yang F, Wang W, Zohbi N, Wang X, Wang D, Zhuang X, Dou Z, Liu D, Song X, Green HN, Liu X, Yao X
Journal of molecular cell biology 2022 Jan 29;13(12):841-852

VTT-006, an anti-mitotic compound, binds to the Ndc80 complex and suppresses cancer cell growth in vitro.

Laine LJ, Mäki-Jouppila JHE, Kutvonen E, Tiikkainen P, Nyholm TKM, Tien JF, Umbreit NT, Härmä V, Kallio L, Davis TN, Asbury CL, Poso A, Gorbsky GJ, Kallio MJ
Oncoscience 2021;8:134-153

Wip1 controls the translocation of the chromosomal passenger complex to the central spindle for faithful mitotic exit.

Zhang X, Park JE, Kim EH, Hong J, Hwang KT, Kim YA, Jang CY
Cellular and molecular life sciences : CMLS 2021 Mar;78(6):2821-2838

Identification of abscission checkpoint bodies as structures that regulate ESCRT factors to control abscission timing.

Strohacker LK, Mackay DR, Whitney MA, Couldwell GC, Sundquist WI, Ullman KS
eLife 2021 Aug 4;10

Cell division requires RNA eviction from condensing chromosomes.

Sharp JA, Perea-Resa C, Wang W, Blower MD
The Journal of cell biology 2020 Nov 2;219(11)

Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence.

Barroso-Vilares M, Macedo JC, Reis M, Warren JD, Compton D, Logarinho E
EMBO reports 2020 May 6;21(5):e49248

BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules.

Huang Y, Lin L, Liu X, Ye S, Yao PY, Wang W, Yang F, Gao X, Li J, Zhang Y, Zhang J, Yang Z, Liu X, Yang Z, Zang J, Teng M, Wang Z, Ruan K, Ding X, Li L, Cleveland DW, Zhang R, Yao X
Cell research 2019 Jul;29(7):562-578

Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.

Achuthankutty D, Thakur RS, Haahr P, Hoffmann S, Drainas AP, Bizard AH, Weischenfeldt J, Hickson ID, Mailand N
The Journal of cell biology 2019 Dec 2;218(12):3943-3953

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Liu S, Kwon M, Mannino M, Yang N, Renda F, Khodjakov A, Pellman D
Nature 2018 Sep;561(7724):551-555

Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.

Weith M, Seiler J, van den Boom J, Kracht M, Hülsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H
Molecular cell 2018 Nov 15;72(4):766-777.e6

Chromosome Segregation Is Biased by Kinetochore Size.

Drpic D, Almeida AC, Aguiar P, Renda F, Damas J, Lewin HA, Larkin DM, Khodjakov A, Maiato H
Current biology : CB 2018 May 7;28(9):1344-1356.e5

Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway.

Xie CM, Lin XT, Wu D, Tan Y, Cheng CHK, Zhang J
Oncotarget 2018 Mar 2;9(17):13783-13795

Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription.

Novais-Cruz M, Alba Abad M, van IJcken WF, Galjart N, Jeyaprakash AA, Maiato H, Ferrás C
eLife 2018 Aug 6;7

Plk1 overexpression induces chromosomal instability and suppresses tumor development.

de Cárcer G, Venkateswaran SV, Salgueiro L, El Bakkali A, Somogyi K, Rowald K, Montañés P, Sanclemente M, Escobar B, de Martino A, McGranahan N, Malumbres M, Sotillo R
Nature communications 2018 Aug 1;9(1):3012

USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.

Skowyra A, Allan LA, Saurin AT, Clarke PR
Cell reports 2018 Apr 17;23(3):852-865

Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.

Shrestha RL, Conti D, Tamura N, Braun D, Ramalingam RA, Cieslinski K, Ries J, Draviam VM
Nature communications 2017 Jul 28;8(1):150

Studying Kinetochore Kinases.

Saurin AT, Kops GJ
Methods in molecular biology (Clifton, N.J.) 2016;1413:333-47

IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells.

Lee S, Jeong AL, Park JS, Han S, Jang CY, Kim KI, Kim Y, Park JH, Lim JS, Lee MS, Yang Y
Cellular and molecular life sciences : CMLS 2016 Sep;73(17):3375-86

The Ska complex promotes Aurora B activity to ensure chromosome biorientation.

Redli PM, Gasic I, Meraldi P, Nigg EA, Santamaria A
The Journal of cell biology 2016 Oct 10;215(1):77-93

Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation.

Mo F, Zhuang X, Liu X, Yao PY, Qin B, Su Z, Zang J, Wang Z, Zhang J, Dou Z, Tian C, Teng M, Niu L, Hill DL, Fang G, Ding X, Fu C, Yao X
Nature chemical biology 2016 Apr;12(4):226-32

Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.

Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg EA, Elowe S
Nature communications 2015 Sep 24;6:8364

ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4.

Thoresen SB, Campsteijn C, Vietri M, Schink KO, Liestøl K, Andersen JS, Raiborg C, Stenmark H
Nature cell biology 2014 Jun;16(6):550-60

TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation.

Ohishi T, Muramatsu Y, Yoshida H, Seimiya H
Molecular and cellular biology 2014 Jul;34(13):2464-78

Negative feedback at kinetochores underlies a responsive spindle checkpoint signal.

Nijenhuis W, Vallardi G, Teixeira A, Kops GJ, Saurin AT
Nature cell biology 2014 Dec;16(12):1257-64

Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres.

Carretero M, Ruiz-Torres M, Rodríguez-Corsino M, Barthelemy I, Losada A
The EMBO journal 2013 Nov 13;32(22):2938-49

Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex.

Rivera T, Ghenoiu C, Rodríguez-Corsino M, Mochida S, Funabiki H, Losada A
The EMBO journal 2012 Mar 21;31(6):1467-79

Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint.

Mackay DR, Makise M, Ullman KS
The Journal of cell biology 2010 Nov 29;191(5):923-31

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Supportive validation

Submitted by: Rockland Immunochemicals, Inc. (provider)
Main image
Experimental details: Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). All lanes : Aurora B (phospho T232) antibody diluted 1:500. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) and with the phosphopeptide immunogen.
Validation comment: Western Blot