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LearnNB500-185
antibody from Novus Biologicals
Targeting: AURKB
Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB500-185 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-185, RRID:AB_10001878
- Product name
- Rabbit Polyclonal Aurora B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Fanconi Anemia C Protein Binds to and Regulates Stathmin-1 Phosphorylation.
Chk2 phosphorylation of survivin-DeltaEx3 contributes to a DNA damage-sensing checkpoint in cancer.
Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1.
Magron A, Elowe S, Carreau M
PloS one 2015;10(10):e0140612
PloS one 2015;10(10):e0140612
Chk2 phosphorylation of survivin-DeltaEx3 contributes to a DNA damage-sensing checkpoint in cancer.
Lopergolo A, Tavecchio M, Lisanti S, Ghosh JC, Dohi T, Faversani A, Vaira V, Bosari S, Tanigawa N, Delia D, Kossenkov AV, Showe LC, Altieri DC
Cancer research 2012 Jul 1;72(13):3251-9
Cancer research 2012 Jul 1;72(13):3251-9
Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1.
Qi W, Tang Z, Yu H
Molecular biology of the cell 2006 Aug;17(8):3705-16
Molecular biology of the cell 2006 Aug;17(8):3705-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B on HeLa whole cell lysate using NB500-185. Immunoprecipitation was also performed using normal rabbit IgG as negative control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Aurora B Antibody [NB500-185] - Detection of human Aurora B by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 (lot NB500-185-2) used at 0.1 ug/ml. Aurora B was also immunoprecipitated by a previous lot of this antibody (lot NB500-185-1). Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aurora B Antibody [NB500-185] - Section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-Aurora B used at a dilution of 1:100. Detection: DAB
