PB9124

antibody from Boster Biological Technology

Targeting: ACE ACE1, CD143, DCP1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9124

Provider

Boster Biological Technology

Product name

Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CD143/ACE detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CD143/ACE information: Molecular Weight: 149715 MW; Subcellular Localization: Angiotensin-converting enzyme, soluble form: Secreted; Tissue Specificity: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Increased abundance of bacteria of the family Muribaculaceae achieved by fecal microbiome transplantation correlates with the inhibition of kidney calcium oxalate stone deposition in experimental rats.

Wang Y, Sun J, Xie S, Zhou Y, Wang T, Liu Z, Li C, Gao L, Pan T
Frontiers in cellular and infection microbiology 2023;13:1145196

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Serum exosomes derived from spontaneously hypertensive rats induce cardiac hypertrophy in vitro and in vivo by increasing autocrine release of angiotensin II in cardiomyocytes.

Yu J, Tang Y, Wang Y, Zhou M, Li Y, Hong J, Li C, Xu B, Guo X, Mao J
Biochemical pharmacology 2023 Apr;210:115462

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Telmisartan protects chronic intermittent hypoxic mice via modulating cardiac renin-angiotensin system activity.

Wang W, Song A, Zeng Y, Chen X, Zhang Y, Shi Y, Lin Y, Luo W
BMC cardiovascular disorders 2018 Jul 3;18(1):133

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Low concentrations of bilirubin inhibit activation of hepatic stellate cells in vitro.

Tang Y, Zhang Q, Zhu Y, Chen G, Yu F
Molecular medicine reports 2017 Apr;15(4):1647-1653

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Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients.

Shuai B, Yang YP, Shen L, Zhu R, Xu XJ, Ma C, Lv L, Zhao J, Rong JH
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 2015 Mar;26(3):1063-71

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of ACE using anti-ACE antibody (PB9124). ACE was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image