RQ4359

antibody from NSJ Bioreagents

Targeting: POLL

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4359

Provider

NSJ Bioreagents

Product name

DNA Polymerase lambda Antibody / POLL

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-2 OS cells with DNA Polymerase lambda antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNA Polymerase lambda antibody.