Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- A301-640A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pol Lambda Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pol Lambda by western blot. Samples: Whole cell lysate (50 µg) from U2OS, GaMG, HeLa, HEK293T, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pol Lambda antibody (Product # A301-640A lot 3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody A301-640A (lot A301-640A-2) used for IP at 6 µg per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody A301-641A. For blotting immunoprecipitated Pol Lambda, A301-640A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pol Lambda by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody A301-640A (lot A301-640A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody (Product # A301-640A; Lot 3) used for IP at 6 µg per reaction. Pol Lambda was also immunoprecipitated by a previous lot of this antibody (Product # A301-640A; Lot 2) and a second antibody against a different epitope of Pol Lambda (Product # A301-641A). For blotting immunoprecipitated Pol Lambda, Product # A301-640A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.