NB100-81665

antibody from Novus Biologicals

Targeting: POLL

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-81665

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-81665, RRID:AB_1145388

Product name

Rabbit Polyclonal DNA Polymerase lambda Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-81665 used for IP at 3 mcg/mg lysate. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81664, which recognizes an upstream epitope.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81665 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81664. For blotting immunoprecipitated Pol Lambda, NB100-81665 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Pol Lambda used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - FFPE section of human prostate nodular hypertrophy. NB100-81665 used at a dilution of 1:250.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DNA Polymerase lambda Antibody [NB100-81665] - Detection of mouse Pol Lambda by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB