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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARG55875 - Provider product page
- Provider
- Arigo
- Product name
- anti-Nucleostemin antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 38-72 (N-terminus) of Human Nucleostemin
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of HeLa, K562, Raji, NCCIT, F9 cell line (from left to right) stained with ARG55875 anti-Nucleostemin antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG55875 anti-Nucleostemin antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). DAPI was used to stain the cell nuclear (blue).
