15060-1-AP

antibody from Invitrogen Antibodies

Targeting: GNL3 C77032, E2IG3, MGC800, NS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15060-1-AP

Provider

Invitrogen Antibodies

Product name

Nucleostemin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TVVFRASTK PKDKGKITKR VKAKKNAAPF RSEVCFGKEG LWKLLGGFQE TCSKAIRVGV IGFPNVGKSS IINSLKQEQM CNVGVSMGLT RSMQVVPLDK QITIIDSPSF IVSPLNSSSA LALRSPASIE VVKPMEAASA ILSQADARQV VLKYTVPGYR NSLEFFTMLA QRRGMHQKGG IPNVEGAAKL LWSEWTGASL AYYCHPPTSW TPPPYFNESI VVDMKSGFNL EELEKNNAQS IRAIKGPHLA NSILFQSSGL TNGIIEEKDI HEELPKRKER KQEEREDDKD SDQETVDEEV DENSSGMFAA EETGEALSEE TTAGEQSTRS FILDKIIEED DAYDFSTDYV (201-549 aa encoded by BC001024)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 15060-1-AP) (GNL3 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Nucleostemin (IP:15060-1-AP, 4ug; Detection:15060-1-AP 1:600) with HeLa cells lysate 4000ug.