H00001655-B01

antibody from Abnova Corporation

Targeting: DDX5 G17P1, HLR1, p68

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001655-B01

Provider

Abnova Corporation

Product name

DDX5 MaxPab mouse polyclonal antibody (B01)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human DDX5 protein.

Antigen sequence

MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFG
NPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTA
QEVETYRRSKEITVRGHNCPKPVLNFYEANFPANV
MDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQT
GSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAP
TRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQ
IRDLERGVEICIATPGRLIDFLECGKTNLRRTTYL
VLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSA
TWPKEVRQLAEDFLKDYIHINIGALELSANHNILQ
IVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVET
KRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLN
EFKHGKAPILIATDVASRGLDVEDVKFVINYDYPN
SSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSD
LISVLREANQAINPKLLQLVEDRGSGRSRGRGGMK
DDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRD
FGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRT
GNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAY
PATAAAPMIGYPMPTGYSQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

DDX17 promotes the production of infectious HIV-1 particles through modulating viral RNA packaging and translation frameshift.

Lorgeoux RP, Pan Q, Le Duff Y, Liang C
Virology 2013 Sep 1;443(2):384-92

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DDX5 expression in transfected 293T cell line (H00001655-T01) by DDX5 MaxPab polyclonal antibody.Lane 1: DDX5 transfected lysate(67.54 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Hela S3 NE.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Raw 264.7.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to DDX5 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol