NBP1-28733

antibody from Novus Biologicals

Targeting: USP35 KIAA1372

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-28733

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-28733, RRID:AB_1913997

Product name

Rabbit Polyclonal USP35 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: USP35 Antibody [NBP1-28733] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP35 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. USP35 was also immunoprecipitated by rabbit anti-USP35 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: USP35 Antibody [NBP1-28733] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP35 antibody used for IP at 10 ug/mg lysate. USP35 was also immunoprecipitated by rabbit anti-USP35 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated USP35, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.