GTX48696

antibody from GeneTex

Targeting: PPP1R13L IASPP, RAI

ELISA

Antibody data

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Validation data

PPP1R13L protein structure - GTX48696 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ?g of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4¢XC. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.