PAB10243

antibody from Abnova Corporation

Targeting: PPP1R13L IASPP, RAI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10243

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10243, RRID:AB_1711610

Product name

PPP1R13L polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13L.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

In vitro effect of iASPP on cell growth of oral tongue squamous cell carcinoma.

Chen Y, Yan W, He S, Chen J, Chen D, Zhang Z, Liu Z, Ding X, Wang A
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2014 Aug;26(4):382-90

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ASPP--Apoptotic specific regulator of p53.

Liu ZJ, Lu X, Zhong S
Biochimica et biophysica acta 2005 Sep 25;1756(1):77-80

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Polymorphisms in RAI and in genes of nucleotide and base excision repair are not associated with risk of testicular cancer.

Laska MJ, Nexø BA, Vistisen K, Poulsen HE, Loft S, Vogel U
Cancer letters 2005 Jul 28;225(2):245-51

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The expression of iASPP in acute leukemias.

Zhang X, Wang M, Zhou C, Chen S, Wang J
Leukemia research 2005 Feb;29(2):179-83

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The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization.

Slee EA, Gillotin S, Bergamaschi D, Royer C, Llanos S, Ali S, Jin B, Trigiante G, Lu X
Oncogene 2004 Dec 2;23(56):9007-16

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RelA-associated inhibitor blocks transcription of human immunodeficiency virus type 1 by inhibiting NF-kappaB and Sp1 actions.

Takada N, Sanda T, Okamoto H, Yang JP, Asamitsu K, Sarol L, Kimura G, Uranishi H, Tetsuka T, Okamoto T
Journal of virology 2002 Aug;76(16):8019-30

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using PPP1R13L polyclonal antibody (Cat # PAB10243) shows detection of a band at ~100 KDa (arrowhead) corresponding to isoform 1 of PPP1R13L in MCF-7 whole cell lysates.Preincubation with immunizing peptide blocks specific band staining (data not shown).Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C.The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of PPP1R13L polyclonal antibody (Cat # PAB10243) shows strong cytoplasmic and membranous staining of myocytes in human heart tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)