Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-77912 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77912, RRID:AB_11033991
- Product name
- Rabbit Polyclonal iASPP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against the human iASPP protein. A BLAST analysis was used to suggest cross reactivity with iASPP proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: iASPP Antibody [NBP1-77912] - Shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: iASPP Antibody [NBP1-77912] - Staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
