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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS1918 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS1918, RRID:AB_1663973
- Product name
- HMGB1 (A164) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 131-180 of Human HMGB1.
- Description
- High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Research suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture, including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, ATF-2, c-Jun and members of the NF°ÙB family to activate transcription. Additional studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 each contain two DNA-binding domains, termed HMG boxes. HMG proteins bind singlestranded DNA but induce conformational changes in double-stranded DNA alone.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of HMGB1 (A164) pAb at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate
