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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB16901 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat HMGB1/HMG-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat HMGB1/HMG-1 in Western blot.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P09429- Isotype
- IgG
- Antibody clone number
- 951420
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury and improved neurological deficits in rats.
Wang D, Liu K, Wake H, Teshigawara K, Mori S, Nishibori M
Scientific reports 2017 Apr 10;7:46243
Scientific reports 2017 Apr 10;7:46243
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat HMGB1/HMG-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, H4-II-E-C3 rat hepatoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB16901) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HMGB1/HMG-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HMGB1/HMG-1 in Human Prostate Cancer Tissue. HMGB1/HMG-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rat Anti-Human/Mouse/Rat HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB16901) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
