Explore
Validate
LearnH00003146-M08
antibody from Novus Biologicals
Targeting: HMGB1
DKFZp686A04236, HMG1, HMG3, SBP-1
Western blot ELISA Immunocytochemistry Immunohistochemistry
Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003146-M08 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003146-M08, RRID:AB_539589
- Product name
- Mouse Monoclonal HMGB1/HMG-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HMGB1 - high-mobility group box 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Knockdown of HMGB1 Suppresses Hypoxia-Induced Mitochondrial Biogenesis in Pancreatic Cancer Cells.
HMGB1 regulates erastin-induced ferroptosis via RAS-JNK/p38 signaling in HL-60/NRAS(Q61L) cells.
Strong and sustained activation of the anticipatory unfolded protein response induces necrotic cell death.
Blockade of HMGB1 Attenuates Diabetic Nephropathy in Mice.
Inhibition of Aurora Kinase A Induces Necroptosis in Pancreatic Carcinoma.
HMGB1 is upregulated in the airways in asthma and potentiates airway smooth muscle contraction via TLR4.
Poly-ADP-ribosylation of HMGB1 regulates TNFSF10/TRAIL resistance through autophagy.
The ferroptosis inducer erastin enhances sensitivity of acute myeloid leukemia cells to chemotherapeutic agents.
Cancer-associated fibroblasts induce high mobility group box 1 and contribute to resistance to doxorubicin in breast cancer cells.
p53/HMGB1 complexes regulate autophagy and apoptosis.
High mobility group box 1 (HMGB1) activates an autophagic response to oxidative stress.
The Receptor for Advanced Glycation End-products (RAGE) protects pancreatic tumor cells against oxidative injury.
The anti-inflammatory activity of HMGB1 A box is enhanced when fused with C-terminal acidic tail.
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
Yang L, Ye F, Zeng L, Li Y, Chai W
OncoTargets and therapy 2020;13:1187-1198
OncoTargets and therapy 2020;13:1187-1198
HMGB1 regulates erastin-induced ferroptosis via RAS-JNK/p38 signaling in HL-60/NRAS(Q61L) cells.
Ye F, Chai W, Xie M, Yang M, Yu Y, Cao L, Yang L
American journal of cancer research 2019;9(4):730-739
American journal of cancer research 2019;9(4):730-739
Strong and sustained activation of the anticipatory unfolded protein response induces necrotic cell death.
Livezey M, Huang R, Hergenrother PJ, Shapiro DJ
Cell death and differentiation 2018 Oct;25(10):1796-1807
Cell death and differentiation 2018 Oct;25(10):1796-1807
Blockade of HMGB1 Attenuates Diabetic Nephropathy in Mice.
Chen X, Ma J, Kwan T, Stribos EGD, Messchendorp AL, Loh YW, Wang X, Paul M, Cunningham EC, Habib M, Alexander IE, Sharland AF, Chadban SJ, Wu H
Scientific reports 2018 May 29;8(1):8319
Scientific reports 2018 May 29;8(1):8319
Inhibition of Aurora Kinase A Induces Necroptosis in Pancreatic Carcinoma.
Xie Y, Zhu S, Zhong M, Yang M, Sun X, Liu J, Kroemer G, Lotze M, Zeh HJ 3rd, Kang R, Tang D
Gastroenterology 2017 Nov;153(5):1429-1443.e5
Gastroenterology 2017 Nov;153(5):1429-1443.e5
HMGB1 is upregulated in the airways in asthma and potentiates airway smooth muscle contraction via TLR4.
Di Candia L, Gomez E, Venereau E, Chachi L, Kaur D, Bianchi ME, Challiss RAJ, Brightling CE, Saunders RM
The Journal of allergy and clinical immunology 2017 Aug;140(2):584-587.e8
The Journal of allergy and clinical immunology 2017 Aug;140(2):584-587.e8
Poly-ADP-ribosylation of HMGB1 regulates TNFSF10/TRAIL resistance through autophagy.
Yang M, Liu L, Xie M, Sun X, Yu Y, Kang R, Yang L, Zhu S, Cao L, Tang D
Autophagy 2015;11(2):214-24
Autophagy 2015;11(2):214-24
The ferroptosis inducer erastin enhances sensitivity of acute myeloid leukemia cells to chemotherapeutic agents.
Yu Y, Xie Y, Cao L, Yang L, Yang M, Lotze MT, Zeh HJ, Kang R, Tang D
Molecular & cellular oncology 2015 Oct-Dec;2(4):e1054549
Molecular & cellular oncology 2015 Oct-Dec;2(4):e1054549
Cancer-associated fibroblasts induce high mobility group box 1 and contribute to resistance to doxorubicin in breast cancer cells.
Amornsupak K, Insawang T, Thuwajit P, O-Charoenrat P, Eccles SA, Thuwajit C
BMC cancer 2014 Dec 15;14:955
BMC cancer 2014 Dec 15;14:955
p53/HMGB1 complexes regulate autophagy and apoptosis.
Livesey KM, Kang R, Vernon P, Buchser W, Loughran P, Watkins SC, Zhang L, Manfredi JJ, Zeh HJ 3rd, Li L, Lotze MT, Tang D
Cancer research 2012 Apr 15;72(8):1996-2005
Cancer research 2012 Apr 15;72(8):1996-2005
High mobility group box 1 (HMGB1) activates an autophagic response to oxidative stress.
Tang D, Kang R, Livesey KM, Zeh HJ 3rd, Lotze MT
Antioxidants & redox signaling 2011 Oct 15;15(8):2185-95
Antioxidants & redox signaling 2011 Oct 15;15(8):2185-95
The Receptor for Advanced Glycation End-products (RAGE) protects pancreatic tumor cells against oxidative injury.
Kang R, Tang D, Livesey KM, Schapiro NE, Lotze MT, Zeh HJ 3rd
Antioxidants & redox signaling 2011 Oct 15;15(8):2175-84
Antioxidants & redox signaling 2011 Oct 15;15(8):2175-84
The anti-inflammatory activity of HMGB1 A box is enhanced when fused with C-terminal acidic tail.
Gong W, Zheng Y, Chao F, Li Y, Xu Z, Huang G, Gao X, Li S, He F
Journal of biomedicine & biotechnology 2010;2010:915234
Journal of biomedicine & biotechnology 2010;2010:915234
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ
Journal of hepatology 2009 Aug;51(2):322-32
Journal of hepatology 2009 Aug;51(2):322-32
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M08), clone 2F6. Lane 1: HMGB1 transfected lysatE (25 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of HMGB1 expression in Hela S3 NE (Cat # L013V3).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Detection limit for recombinant GST tagged HMGB1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (2F6) [H00003146-M08] - Analysis of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
