Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00055510-M07 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055510-M07, RRID:AB_1145339
- Product name
- Mouse Monoclonal DDX43 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DDX43 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 (3G12)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Arresting of miR-186 and releasing of H19 by DDX43 facilitate tumorigenesis and CML progression.
Lin J, Ma JC, Yang J, Yin JY, Chen XX, Guo H, Wen XM, Zhang TJ, Qian W, Qian J, Deng ZQ
Oncogene 2018 May;37(18):2432-2443
Oncogene 2018 May;37(18):2432-2443
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX43 Antibody (3G12) [H00055510-M07] - Analysis of DDX43 expression in transfected 293T cell line by DDX43 monoclonal antibody (M07), clone 3G12. Lane 1: DDX43 transfected lysatE (72.9 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DDX43 Antibody (3G12) [H00055510-M07] - Detection limit for recombinant GST tagged DDX43 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DDX43 Antibody (3G12) [H00055510-M07] - Analysis of monoclonal antibody to DDX43 on HeLa cell. Antibody concentration 10 ug/ml