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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- 17591-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX43 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KPTPIQSQA WPIVLQGIDL IGVAQTGTGK TLCYLMPGFI HLVLQPSLKG QRNRPGMLVL TPTRELALQV EGECCKYSYK GLRSVCVYGG GNRDEQIEEL KKGVDIIIAT PGRLNDLQMS NFVNLKNITY LVLDEADKML DMGFEPQIMK ILLDVRPDRQ TVMTSATWPH SVHRLAQSYL KEPMIVYVGT LDLVAVSSVK QNIIVTTEEE KWSHMQTFLQ SMSSTDKVIV FVSRKAVADH LSSDLILGNI SVESLHGDRE QRDREKALEN FKTGKVRILI ATDLASRGLD VHDVTHVYNF DFPRNIEEYV HRIGRTGRAG RTGVSITTLT RNDWRVASEL INILERANQS IPEELVSMAE RFKAHQQKRE MERKMERPQG RPKKFH (264-648 aa encoded by BC066938)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 17591-1-AP (DDX43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 17591-1-AP (DDX43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 40x lens).
