Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 11402-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11402-1-AP, RRID:AB_2096966
- Product name
- EEF1A1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated EEF1A1 antibody (Cat. #11402-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.
Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.
A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.
Opposing roles of C/EBPα and eEF1A1 in Sp1-regulated miR-122 transcription.
A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.
RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.
Novel nannocystin A analogues as anticancer therapeutics: Synthesis, biological evaluations and structure-activity relationship studies.
Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.
Contradictory mRNA and protein misexpression of EEF1A1 in ductal breast carcinoma due to cell cycle regulation and cellular stress.
Acetylation of Cytidine in mRNA Promotes Translation Efficiency.
Single-cell gene expression analysis reveals diversity among human spermatogonia.
The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.
Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.
Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.
Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.
Asamitsu S, Yabuki Y, Ikenoshita S, Kawakubo K, Kawasaki M, Usuki S, Nakayama Y, Adachi K, Kugoh H, Ishii K, Matsuura T, Nanba E, Sugiyama H, Fukunaga K, Shioda N
Science advances 2021 Jan;7(3)
Science advances 2021 Jan;7(3)
Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.
Qin S, Ye L, Zheng Y, Gao J
FEBS letters 2021 Feb;595(4):507-520
FEBS letters 2021 Feb;595(4):507-520
A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.
Hou Y, Lu H, Li J, Guan Z, Zhang J, Zhang W, Yin C, Sun L, Zhang Y, Jiang H
Cell chemical biology 2021 Aug 30;
Cell chemical biology 2021 Aug 30;
Opposing roles of C/EBPα and eEF1A1 in Sp1-regulated miR-122 transcription.
Zeng C, Sang Y, Wang FY, Zhuang SM
RNA biology 2020 Feb;17(2):202-210
RNA biology 2020 Feb;17(2):202-210
A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.
Bosutti A, Kalaja O, Zanconati F, Dapas B, Grassi G, Passamonti S, Scaggiante B
Journal of pharmaceutical and biomedical analysis 2019 Nov 30;176:112814
Journal of pharmaceutical and biomedical analysis 2019 Nov 30;176:112814
RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.
Lin X, Chai G, Wu Y, Li J, Chen F, Liu J, Luo G, Tauler J, Du J, Lin S, He C, Wang H
Nature communications 2019 May 6;10(1):2065
Nature communications 2019 May 6;10(1):2065
Novel nannocystin A analogues as anticancer therapeutics: Synthesis, biological evaluations and structure-activity relationship studies.
Liu Q, Yang X, Ji J, Zhang SL, He Y
European journal of medicinal chemistry 2019 May 15;170:99-111
European journal of medicinal chemistry 2019 May 15;170:99-111
Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.
Deng JH, Chen HY, Huang C, Yan JM, Yin Z, Zhang XL, Pan Q
Pathogens and disease 2019 Feb 1;77(1)
Pathogens and disease 2019 Feb 1;77(1)
Contradictory mRNA and protein misexpression of EEF1A1 in ductal breast carcinoma due to cell cycle regulation and cellular stress.
Lin CY, Beattie A, Baradaran B, Dray E, Duijf PHG
Scientific reports 2018 Sep 17;8(1):13904
Scientific reports 2018 Sep 17;8(1):13904
Acetylation of Cytidine in mRNA Promotes Translation Efficiency.
Arango D, Sturgill D, Alhusaini N, Dillman AA, Sweet TJ, Hanson G, Hosogane M, Sinclair WR, Nanan KK, Mandler MD, Fox SD, Zengeya TT, Andresson T, Meier JL, Coller J, Oberdoerffer S
Cell 2018 Dec 13;175(7):1872-1886.e24
Cell 2018 Dec 13;175(7):1872-1886.e24
Single-cell gene expression analysis reveals diversity among human spermatogonia.
Neuhaus N, Yoon J, Terwort N, Kliesch S, Seggewiss J, Huge A, Voss R, Schlatt S, Grindberg RV, Schöler HR
Molecular human reproduction 2017 Feb 10;23(2):79-90
Molecular human reproduction 2017 Feb 10;23(2):79-90
The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.
Liu X, Chen L, Ge J, Yan C, Huang Z, Hu J, Wen C, Li M, Huang D, Qiu Y, Hao H, Yuan R, Lei J, Yu X, Shao J
Cancer research 2016 Aug 15;76(16):4897-907
Cancer research 2016 Aug 15;76(16):4897-907
Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.
Lu Y, Wang J, Dapeng C, Wu D, Cai G, Chen X
BMC nephrology 2016 Apr 1;17:39
BMC nephrology 2016 Apr 1;17:39
Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.
Mizuno K, Kojima Y, Kurokawa S, Maruyama T, Sasaki S, Kohri K, Hayashi Y
The Journal of urology 2009 Mar;181(3):1330-7; discussion 1337
The Journal of urology 2009 Mar;181(3):1330-7; discussion 1337
Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.
Bao H, Song P, Liu Q, Liu Y, Yun D, Saiyin H, Du R, Zhang Y, Fan H, Yang P, Chen X
Proteomics. Clinical applications 2009 Jun;3(6):705-19
Proteomics. Clinical applications 2009 Jun;3(6):705-19
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11402-1-AP(EEF1A1 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using EEF1A1 antibody 11402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 11402-1-AP(EEF1A1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 11402-1-AP(EEF1A1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue