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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [4]
- ELISA [1]
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- Product number
- H00283455-M08 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00283455-M08, RRID:AB_540100
- Product name
- Mouse Monoclonal KSR2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KSR2 - kinase suppressor of ras 2
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references G protein-coupled estrogen receptor 1 (GPER1)/GPR30 increases ERK1/2 activity through PDZ motif-dependent and -independent mechanisms.
Kinase suppressor of Ras 2 (KSR2) regulates tumor cell transformation via AMPK.
KSR2 is an essential regulator of AMP kinase, energy expenditure, and insulin sensitivity.
Gonzalez de Valdivia E, Broselid S, Kahn R, Olde B, Leeb-Lundberg LMF
The Journal of biological chemistry 2017 Jun 16;292(24):9932-9943
The Journal of biological chemistry 2017 Jun 16;292(24):9932-9943
Kinase suppressor of Ras 2 (KSR2) regulates tumor cell transformation via AMPK.
Fernandez MR, Henry MD, Lewis RE
Molecular and cellular biology 2012 Sep;32(18):3718-31
Molecular and cellular biology 2012 Sep;32(18):3718-31
KSR2 is an essential regulator of AMP kinase, energy expenditure, and insulin sensitivity.
Costanzo-Garvey DL, Pfluger PT, Dougherty MK, Stock JL, Boehm M, Chaika O, Fernandez MR, Fisher K, Kortum RL, Hong EG, Jun JY, Ko HJ, Schreiner A, Volle DJ, Treece T, Swift AL, Winer M, Chen D, Wu M, Leon LR, Shaw AS, McNeish J, Kim JK, Morrison DK, Tschöp MH, Lewis RE
Cell metabolism 2009 Nov;10(5):366-78
Cell metabolism 2009 Nov;10(5):366-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KSR2 Antibody (1G4) [H00283455-M08] - Western Blot analysis of KSR2 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KSR2 Antibody (1G4) [H00283455-M08] - Western blot analysis of KSR2 over-expressed 293 cell line, cotransfected with KSR2 Validated Chimera RNAi or non-transfected control. Blot probed with H00283455-M08. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KSR2 Antibody (1G4) [H00283455-M08] - Analysis of KSR2 expression in transfected 293T cell line by KSR2 monoclonal antibody (M08), clone 1G4. Lane 1: KSR2 transfected lysate (Predicted MW: 93.6 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KSR2 Antibody (1G4) [H00283455-M08] - Analysis of KSR2 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: KSR2 Antibody (1G4) [H00283455-M08] - Detection limit for recombinant GST tagged KSR2 is approximately 0.03ng/ml as a capture antibody.
