Explore
Validate
LearnLS-C331306
antibody from LSBio
Targeting: KDM1A
AOF2, BHC110, KDM1, KIAA0601, LSD1
Western blot
Immunocytochemistry
Immunoprecipitation Immunohistochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C331306 - Provider product page
- Provider
- LSBio
- Product name
- KDM1A / LSD1 Antibody LS-C331306
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lysates.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using KDM1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and KDM1A knockout (KO) HeLa cells, using KDM1A antibodyat 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody. Western blot was performed from the immunoprecipitate using KDM1A antibodyat a dilition of 1:500.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody. Western blot was performed from the immunoprecipitate using KDM1A antibodyat a dilition of 1:500.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat kidney tissue.
