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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB5530 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5530, RRID:AB_10549400
- Product name
- AOF2 monoclonal antibody, clone 1B2E5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant AOF2.
- Isotype
- IgG
- Antibody clone number
- 1B2E5
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of LSD1 histone demethylase activity by its associated factors.
LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
Shi YJ, Matson C, Lan F, Iwase S, Baba T, Shi Y
Molecular cell 2005 Sep 16;19(6):857-64
Molecular cell 2005 Sep 16;19(6):857-64
LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
Metzger E, Wissmann M, Yin N, Müller JM, Schneider R, Peters AH, Günther T, Buettner R, Schüle R
Nature 2005 Sep 15;437(7057):436-9
Nature 2005 Sep 15;437(7057):436-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using AOF2 monoclonal antibody, clone 1B2E5 (Cat # MAB5530) against truncated AOF2 recombinant protein (Lane 1), HeLa (Lane 2) and Jurkat (Lane 3) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung carcinoma and kidney carcinoma tissue, showing nuclear localization using AOF2 monoclonal antibody, clone 1B2E5 (Cat # MAB5530) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
