Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- PAB14121 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14121, RRID:AB_10548275
- Product name
- AOF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AOF2.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 40 ug) and AOF2 polyclonal antibody (Cat # PAB14121) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of AOF2 polyclonal antibody (Cat # PAB14121), crude serum and Flow Through in antigen coated wells.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 20,000.
- Validation comment
- ELISA