Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 20813-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TQGKLEEKL QELEANPPSD VYLSSRDRQI LDWHFANLEF ANATPLSTLS LKHWDQDDDF EFTGSHLTVR NGYSCVPVAL AEGLDIKLNT AVRQVRYTAS GCEVIAVNTR STSQTFIYKC DAVLCTLPLG VLKQQPPAVQ FVPPLPEWKT SAVQRMGFGN LNKVVLCFDR VFWDPSVNLF GHVGSTTASR GELFLFWNLY KAPILLALVA GEAAGIMENI SDDVIVGRCL AILKGIFGSS AVPQPKETVV SRWRADPWAR GSYSYVAAGS SGNDYDLMAQ PITPGPSIPG APQPIPRLFF AGEHTIRNYP ATVHGALLSG LREAGRIADQ FLGAMYTLPR QATPGVPAQQ SPSM (524-876 aa encoded by B C040194)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293T cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 20813-1-AP (KDM1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 20813-1-AP ( KDM1 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human KDM1 (Product # 20813-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-KDM1 (IP:20813-1-AP, 4ug; Detection:20813-1-AP 1:600) with HeLa cells lysate 2000ug.