Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-22393 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFMBT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, Jurkat, HeLa.
- Concentration
- 0.6 mg/mL
Submitted references SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.
Pan R, Yu D, Hu J, Yang X, Wang C, Zhang L, Xue P, Sun J, Zhang X, Cai W
Cell death discovery 2022 May 16;8(1):263
Cell death discovery 2022 May 16;8(1):263
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SFMBT1 Polyclonal Antibody (Product # PA5-22393). Sample (30 µg of whole cell lysate). Lane A: IMR32. 7.5% SDS PAGE. SFMBT1 Polyclonal Antibody (Product # PA5-22393) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Distribution of SFMBT1 in colorectal cancer (CRC) tumor tissues and cells. A Western blot analysis of SFMBT1 protein levels in normal and tumor tissues. ( n = 6) ( B ) Expression of SFMBT1 in normal and CRC tumor tissues were examined by immunohistochemistry. C Expression of SFMBT1 in CRC tissue microarrays. D , E TCGA data shows the expression of SFMBT1 in tumor tissues and normal tissues or in different cancer stages. F , G qRT-PCR ( F ) and western blot analysis ( G ) detect SFMBT1 mRNA and protein in human colorectal epithelial cells CCD18C0 and CRC cell lines HCT-116, SW620 and SW480.