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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- MA5-32980 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-VDAC Recombinant Rabbit Monoclonal Antibody (G8F6)
- Antibody type
- Other
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: LO2, A-549, A-431, Raji, mouse heart, mouse kidney, mouse brain, rat heart, rat brain; Cellular Location: Cell membrane, Membrane raft, Mitochondrion outer membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G8F6
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using VDAC1 Monoclonal antibody (Product # MA5-32980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of A549 (Lane 1), Raji (Lane 2), and Mouse brain (Lane 3). The blot was probed with Anti-VDAC1 Monoclonal Antibody (Product # MA5-32980, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 36 kDa band corresponding to VDAC1 was observed in all cell line(s) and mouse tissue tested along with an uncharacterized band at ~78 kDa in human cell lines.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of VDAC in extracts of various cell lines using VDAC Monoclonal Antibody (G8F6) (Product # MA5-32980) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using VDAC1 Monoclonal antibody (Product # MA5-32980) at dilution of 1:100. Blue: DAPI for nuclear staining.
