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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-27547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNRPD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (98%), Dog (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.
Hartmann H, Hornburg D, Czuppa M, Bader J, Michaelsen M, Farny D, Arzberger T, Mann M, Meissner F, Edbauer D
Life science alliance 2018 May;1(2):e201800070
Life science alliance 2018 May;1(2):e201800070
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNRPD2 Polyclonal Antibody (Product # PA5-27547). Sample (30 µg of whole cell lysate). Lane A: A549 . 15% SDS PAGE. SNRPD2 Polyclonal Antibody (Product # PA5-27547) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPD2 Polyclonal Antibody detects SNRPD2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: SNRPD2 protein stained by SNRPD2 Polyclonal Antibody (Product # PA5-27547) diluted at 1:2,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SNRPD2 (Product # PA5-27547) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPD2 Polyclonal Antibody detects SNRPD2 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SNRPD2 Polyclonal Antibody (Product # PA5-27547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPD2 Polyclonal Antibody detects SNRPD2 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SNRPD2 Polyclonal Antibody (Product # PA5-27547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPD2 Polyclonal Antibody detects SNRPD2 protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. SNRPD2 Polyclonal Antibody (Product # PA5-27547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPD2 Polyclonal Antibody detects SNRPD2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SNRPD2 Polyclonal Antibody (Product # PA5-27547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
