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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- GTX11302 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX11302, RRID:AB_381291
- Product name
- alpha Tubulin antibody [DM 1A]
- Antibody type
- Monoclonal
- Antigen
- Purified chick brain tubulin.
- Description
- Mouse monoclonal [DM 1A] to alpha Tubulin
- Reactivity
- Mouse, Bovine, Chicken/Avian, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- raw ascites
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Submitted references The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.
A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress.
Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.
Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Downregulation of FIP200 induces apoptosis of glioblastoma cells and microvascular endothelial cells by enhancing Pyk2 activity.
Nuclear export and import of human hepatitis B virus capsid protein and particles.
Chou SF, Tsai ML, Huang JY, Chang YS, Shih C
PLoS pathogens 2015 Oct;11(10):e1005123
PLoS pathogens 2015 Oct;11(10):e1005123
A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress.
Wei PC, Wang ZF, Lo WT, Su MI, Shew JY, Chang TC, Lee WH
Nucleic acids research 2013 Feb 1;41(3):1533-43
Nucleic acids research 2013 Feb 1;41(3):1533-43
Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.
Chang YC, Yu YH, Shew JY, Lee WJ, Hwang JJ, Chen YH, Chen YR, Wei PC, Chuang LM, Lee WH
EMBO molecular medicine 2013 Aug;5(8):1165-79
EMBO molecular medicine 2013 Aug;5(8):1165-79
Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Wei PC, Lo WT, Su MI, Shew JY, Lee WH
Nucleic acids research 2012 Jan;40(1):323-32
Nucleic acids research 2012 Jan;40(1):323-32
Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Wei PC, Hsieh YH, Su MI, Jiang X, Hsu PH, Lo WT, Weng JY, Jeng YM, Wang JM, Chen PL, Chang YC, Lee KF, Tsai MD, Shew JY, Lee WH
Molecular cell 2012 Dec 14;48(5):747-59
Molecular cell 2012 Dec 14;48(5):747-59
Downregulation of FIP200 induces apoptosis of glioblastoma cells and microvascular endothelial cells by enhancing Pyk2 activity.
Wang D, Olman MA, Stewart J Jr, Tipps R, Huang P, Sanders PW, Toline E, Prayson RA, Lee J, Weil RJ, Palmer CA, Gillespie GY, Liu WM, Pieper RO, Guan JL, Gladson CL
PloS one 2011;6(5):e19629
PloS one 2011;6(5):e19629
Nuclear export and import of human hepatitis B virus capsid protein and particles.
Li HC, Huang EY, Su PY, Wu SY, Yang CC, Lin YS, Chang WC, Shih C
PLoS pathogens 2010 Oct 28;6(10):e1001162
PLoS pathogens 2010 Oct 28;6(10):e1001162
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-£\-Tubulin (GTX11302). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjμgate and AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-£\-Tubulin, clone no. DM 1A (GTX11302) and Anti-Mouse IgG-lissamine-rhodamine conjμgate. Note the large bundles of microtubules which form a well-developed cytophyrigeal basket.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-£\-Tubulin, clone no. DM1A (GTX11302) with Anti-Mouse-Alexa 488 (green) and DAPI (blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human skin fibroblasts stained with Monoclonal Anti-£\-Tubulin, clone No. DM1A (GTX11302).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human heart sections using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
- Validation comment
- IB
