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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-257 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-257, RRID:AB_2197301
- Product name
- Goat Polyclonal SDS3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Allelic variation and differential expression of the mSIN3A histone deacetylase complex gene Arid4b promote mammary tumor growth and metastasis.
Breast cancer metastasis suppressor 1 (BRMS1) is stabilized by the Hsp90 chaperone.
Cdk5/p35 phosphorylates mSds3 and regulates mSds3-mediated repression of transcription.
Winter SF, Lukes L, Walker RC, Welch DR, Hunter KW
PLoS genetics 2012 May;8(5):e1002735
PLoS genetics 2012 May;8(5):e1002735
Breast cancer metastasis suppressor 1 (BRMS1) is stabilized by the Hsp90 chaperone.
Hurst DR, Mehta A, Moore BP, Phadke PA, Meehan WJ, Accavitti MA, Shevde LA, Hopper JE, Xie Y, Welch DR, Samant RS
Biochemical and biophysical research communications 2006 Oct 6;348(4):1429-35
Biochemical and biophysical research communications 2006 Oct 6;348(4):1429-35
Cdk5/p35 phosphorylates mSds3 and regulates mSds3-mediated repression of transcription.
Li Z, David G, Hung KW, DePinho RA, Fu AK, Ip NY
The Journal of biological chemistry 2004 Dec 24;279(52):54438-44
The Journal of biological chemistry 2004 Dec 24;279(52):54438-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SDS3 Antibody [NB100-257] - Detection of Human and Mouse SDS3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SDS3 antibody NB100-257 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SDS3 Antibody [NB100-257] - RIPA whole cell extract (50 ug) from HeLa cells. Antibody used at the concentrations that are indicated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SDS3 Antibody [NB100-257] - Detection of human SDS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SDS3 antibody NB100-257 (lot 2) used for IP at 6 ug per reaction. SDS3 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-SDS3 antibody NB100-93318. For blotting immunoprecipitated SDS3, NB100-257 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
