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LearnA301-348A
antibody from Invitrogen Antibodies
Targeting: PCNT
KEN, KIAA0402, PCN, PCNT2, PCNTB, SCKL4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- A301-348A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pericentrin/Kendrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pericentrin-Kendrin by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrin antibody (Product # A301-348A lot 4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 second.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pericentrin/Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pericentrin/Kendrin antibody A301-348A (lot A301-348A-3) used for IP at 6 µg per reaction. Pericentrin/Kendrin was also immunoprecipitated by a previous lot of this antibody (lot A301-348A-1). For blotting immunoprecipitated Pericentrin/Kendrin, A301-348A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pericentrin/Kendrin by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin antibody A301-348A (lot A301-348A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pericentrin/Kendrin by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin (Cat. No.A301-348A Lot2) used at a dilution of 1:1,000 (1 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain. DAPI (blue) and Phalloidin (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pericentrin-Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pericentrin-Kendrin antibody (Product # A301-348A lot 4) used for IP at 6 µg per reaction. Pericentrin-Kendrin was also immunoprecipitated by a previous lot of this antibody (Product # A301-348A lot 3) and another rabbit anti-Pericentrin-Kendrin antibody (BL5517). For blotting immunoprecipitated Pericentrin-Kendrin (Product # A301-348A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
