GTX83510

antibody from GeneTex

Targeting: TKT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX83510

Provider

GeneTex

Proper citation

GeneTex Cat#GTX83510, RRID:AB_10726982

Product name

Transketolase antibody [5H3]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TKT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TKT.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TKT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-TKT mouse monoclonal antibody (GTX83510) immunofluorescent staining of COS7 cells transiently transfected with TKT

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells, using anti-TKT antibody(GTX83510),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).