TA500892

antibody from OriGene

Targeting: TKT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500892

Provider

OriGene

Proper citation

OriGene Cat#TA500892, RRID:AB_11126263

Product name

Anti-TKT (Transketolase) mouse monoclonal antibody, clone OTI5H3 (formerly 5H3)

Antibody type

Monoclonal

Description

Anti-TKT (Transketolase) mouse monoclonal antibody, clone OTI5H3 (formerly 5H3)

Host

Mouse

Conjugate

Unconjugated

Epitope

TKT

Isotype

IgG

Antibody clone number

OTI5H3

Vial size

100 µl

Concentration

0.85 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TKT (RC203497, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TKT.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TKT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-TKT mouse monoclonal antibody (TA500892) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TKT(RC203497).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-TKT antibody(TA500892),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

Validation comment

FC