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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- 66016-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transketolase Monoclonal Antibody (3E2F3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: IYSQIQSKK KILATPPQED APSVDIANIR MPSLPSYKVG DKIATRKAYG QALAKLGHAS DRIIALDGDT KNSTFSEIFK KEHPDRFIEC YIAEQNMVSI AVGCATRNRT VPFCSTFAAF FTRAFDQIRM AAISESNINL CGSHCGVSIG EDGPSQMALE DLAMFRSVPT STVFYPSDGV ATEKAVELAA NTKGICFIRT SRPENAIIYN NNEDFQVGQA KVVLKSKDDQ VTVIGAGVTL HEALAAAELL KKEKINIRVL DPFTIKPLDR KLILDSARAT KGRILTVEDH YYEGGIGEAV SSAVVGEPGI TVTHLAVNRV PRSGKPAELL KMFGIDRDAI AQAVRGLITK A (274-623 aa encoded by BC009970)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E2F3
- Vial size
- 150 µL
- Concentration
- 1.01 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-IG (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 66016-1-IG (Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 66016-1-IG (Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 66016-1-Ig (Transketolase antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 66016-1-Ig (Transketolase antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 66016-1-IG (Transketolase antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 66016-1-IG (Transketolase antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug Transketolase antibody (66016-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.
