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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 25183-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GBF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ASIYSSWAE EQRHLETGGQ KIEADSRTLW AHCWCPLLQG IACLCCDARR QVRMQALTYL QRALLVHDLQ KLDALEWESC FNKVLFPLLT KLLENISPAD VGGMEETRMR ASTLLSKVFL QHLSPLLSLS TFAALWLTIL DFMDKYMHAG SSDLLSEAIP ESLKNMLLVM DTAEIFHSAD ARGGGPSALW EITWERIDCF LPHLRDELFK QTVIQDPMPM EPQGQKPLAS AHLTSAAGDT RTPGHPPPPE IPSELGACDF EKPESPRAAS SSSPGSPVAS SPSRLSPTPD GPPPLAQPPL ILQPLASPLQ VGVPPMTLPI ILNPALIEAT SPVPLLATPR PTDPIPTSEV N (1510-1859 aa encoded by BC117681)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25183-1-AP ( GBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 25183-1-AP (GBF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP ( GBF1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP ( GBF1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
