15391-1-AP

antibody from Invitrogen Antibodies

Targeting: BRCC3 BRCC36, C6.1A, CXorf53

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15391-1-AP

Provider

Invitrogen Antibodies

Product name

BRCC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LESDAFLVC LNHALSTEKE EVMGLCIGEL NDDTRSDSKF AYTGTEMRTV AEKVDAVRIV HIHSVIILRR SDKRKDRVEI SPEQLSAAST EAERLAELTG RPMRVVGWYH SHPHITVWPS HVDVRTQAMY QMMDQGFVGL IFSCFIEDKN TKTGRVLYTC FQSIQAQKSS ESLHGPRDFW SSSQHISIEG QKEEERYERI EIPIHIVPHV TIGKVCLESA VELPKILCQE EQDAYRRIHS LTHLDSVTKI HNGSVFTKNL CSQMSAVSGP LLQWLEDRLE QNQQHLQELQ QEKEELMQEL SSLE (14-316 aa encoded by BC002999)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15391-1-AP (BRCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15391-1-AP (BRCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 15391-1-AP (BRCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15391-1-AP (BRCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COS-7 cells were subjected to SDS PAGE followed by western blot with 15391-1-AP (BRCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 15391-1-AP (BRCC3 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 15391-1-AP (BRCC3 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 15391-1-AP (BRCC3 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 15391-1-AP (BRCC3 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using (Product # 15391-1-AP) (BRCC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BRCC3 (IP:15391-1-AP, 4ug; Detection:15391-1-AP 1:400) with mouse heart tissue lysate 4000ug.