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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-20602 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TET3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Bovine, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1.
Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter.
α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.
Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury.
Ma M, Zhou QJ, Xiong Y, Li B, Li XT
American journal of translational research 2018;10(1):16-39
American journal of translational research 2018;10(1):16-39
Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter.
Yang R, Yu T, Kou X, Gao X, Chen C, Liu D, Zhou Y, Shi S
Nature communications 2018 Jun 1;9(1):2143
Nature communications 2018 Jun 1;9(1):2143
α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.
Atlante S, Visintin A, Marini E, Savoia M, Dianzani C, Giorgis M, Sürün D, Maione F, Schnütgen F, Farsetti A, Zeiher AM, Bertinaria M, Giraudo E, Spallotta F, Cencioni C, Gaetano C
Cell death & disease 2018 Jul 9;9(7):756
Cell death & disease 2018 Jul 9;9(7):756
Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury.
Le Bras A, Yu B, Issa Bhaloo S, Hong X, Zhang Z, Hu Y, Xu Q
Arteriosclerosis, thrombosis, and vascular biology 2018 Jan;38(1):232-244
Arteriosclerosis, thrombosis, and vascular biology 2018 Jan;38(1):232-244
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-20602] - Mouse colon. TET3 antibody [C3], C-term dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-20602] - Mouse colon. TET3 stained by TET3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min.
