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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 12867-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTSGNGNSA SSIAGTAPQN GENKPPQAIV KPQILTHVIE GFVIQEGAEP FPVGRSSLLV GNLKKKYAQG FLPEKLPQQD HTTTTDSEME EPYLQESKEE GAPLKLKCEL CGRVDFAYKF KRSKRFCSMA CAKRYNVGCT KRVGLFHSDR SKLQKAGAAT HNRRRASKAS LPPLTKDTKK QPTGTVPLSV TAALQLTHSQ EDSSRCSDNS SYEEPLSPIS ASSSTSRRRQ GQRDLELPDM HMRDLVGMGH HFLPSEPTKW NVEDVYEFIR SLPGCQEIEE EFRAQEIDGQ ALLLLKEDHL MSAMNIKLGP ALKIYARISM LKDS (1-323 aa encoded by BC028396)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP ( PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:500) with HL-60 cells lysate 4000 ug.
