Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ7166 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EED Antibody / Embryonic ectoderm development protein
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Embryonic ectoderm development protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human HepG2, 3) human K562, 4) human Caco-2, 5) human SiHa, 6) human RT4, 7) rat brain, 8) rat heart, 9) rat testis, 10) rat C6, 11) mouse brain and 12) mouse NIH 3T3 cell lysate with Embryonic ectoderm development protein antibody. Expected molecular weight: 30~65 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A549 cells with Embryonic ectoderm development protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Embryonic ectoderm development protein antibody.