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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF5827 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse EED Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse EED in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O75530- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Differential expression profiles of conserved Snail transcription factors in the mouse testis.
Canonical PRC2 function is essential for mammary gland development and affects chromatin compaction in mammary organoids.
Micati DJ, Hime GR, McLaughlin EA, Abud HE, Loveland KL
Andrology 2018 Mar;6(2):362-373
Andrology 2018 Mar;6(2):362-373
Canonical PRC2 function is essential for mammary gland development and affects chromatin compaction in mammary organoids.
Michalak EM, Milevskiy MJG, Joyce RM, Dekkers JF, Jamieson PR, Pal B, Dawson CA, Hu Y, Orkin SH, Alexander WS, Lindeman GJ, Smyth GK, Visvader JE
PLoS biology 2018 Aug;16(8):e2004986
PLoS biology 2018 Aug;16(8):e2004986
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EED by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT-29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5827) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EED at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
