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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [30]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AF645 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Wnt-5a Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt-5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22725- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis-Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells.
Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway.
ERAD-dependent control of the Wnt secretory factor Evi.
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD.
Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice.
Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.
IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling.
Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation.
WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation.
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development.
TGF-β-activated kinase 1 (TAK1) signaling regulates TGF-β-induced WNT-5A expression in airway smooth muscle cells via Sp1 and β-catenin.
Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway.
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
WNT5A signaling contributes to Aβ-induced neuroinflammation and neurotoxicity.
Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence.
Evidence for altered Wnt signaling in psoriatic skin.
Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons.
Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10.
Wnt5a is expressed in murine and human atherosclerotic lesions.
Dickkopf-1 is a master regulator of joint remodeling.
Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation.
Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo.
Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.
Xu J, Li X, Cole A, Sherman Z, Du W
Stem cells (Dayton, Ohio) 2018 May;36(5):785-795
Stem cells (Dayton, Ohio) 2018 May;36(5):785-795
Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway.
Han C, Li J, Wang C, Ouyang H, Ding X, Liu Y, Chen S, Luo L
Investigative ophthalmology & visual science 2018 Jul 2;59(8):3449-3460
Investigative ophthalmology & visual science 2018 Jul 2;59(8):3449-3460
ERAD-dependent control of the Wnt secretory factor Evi.
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M
The EMBO journal 2018 Feb 15;37(4)
The EMBO journal 2018 Feb 15;37(4)
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD.
Baarsma HA, Skronska-Wasek W, Mutze K, Ciolek F, Wagner DE, John-Schuster G, Heinzelmann K, Günther A, Bracke KR, Dagouassat M, Boczkowski J, Brusselle GG, Smits R, Eickelberg O, Yildirim AÖ, Königshoff M
The Journal of experimental medicine 2017 Jan;214(1):143-163
The Journal of experimental medicine 2017 Jan;214(1):143-163
Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice.
Chen CM, Orefice LL, Chiu SL, LeGates TA, Hattar S, Huganir RL, Zhao H, Xu B, Kuruvilla R
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 24;114(4):E619-E628
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 24;114(4):E619-E628
Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.
Skaria T, Bachli E, Schoedon G
Cell adhesion & migration 2017 Jan 2;11(1):24-38
Cell adhesion & migration 2017 Jan 2;11(1):24-38
IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling.
Skaria T, Burgener J, Bachli E, Schoedon G
PloS one 2016;11(5):e0156002
PloS one 2016;11(5):e0156002
Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation.
Ramírez VT, Ramos-Fernández E, Henríquez JP, Lorenzo A, Inestrosa NC
The Journal of biological chemistry 2016 Sep 2;291(36):19092-107
The Journal of biological chemistry 2016 Sep 2;291(36):19092-107
WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation.
Rider V, Talbott A, Bhusri A, Krumsick Z, Foster S, Wormington J, Kimler BF
The Journal of endocrinology 2016 May;229(2):197-207
The Journal of endocrinology 2016 May;229(2):197-207
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development.
Uchiyama K, Sakiyama T, Hasebe T, Musch MW, Miyoshi H, Nakagawa Y, He TC, Lichtenstein L, Naito Y, Itoh Y, Yoshikawa T, Jabri B, Stappenbeck T, Chang EB
Scientific reports 2016 Aug 26;6:32094
Scientific reports 2016 Aug 26;6:32094
TGF-β-activated kinase 1 (TAK1) signaling regulates TGF-β-induced WNT-5A expression in airway smooth muscle cells via Sp1 and β-catenin.
Kumawat K, Menzen MH, Slegtenhorst RM, Halayko AJ, Schmidt M, Gosens R
PloS one 2014;9(4):e94801
PloS one 2014;9(4):e94801
Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
Dawes LJ, Sugiyama Y, Lovicu FJ, Harris CG, Shelley EJ, McAvoy JW
Developmental biology 2014 Jan 15;385(2):291-303
Developmental biology 2014 Jan 15;385(2):291-303
WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
Ekström EJ, Bergenfelz C, von Bülow V, Serifler F, Carlemalm E, Jönsson G, Andersson T, Leandersson K
Molecular cancer 2014 Apr 26;13:88
Molecular cancer 2014 Apr 26;13:88
A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
Florian MC, Nattamai KJ, Dörr K, Marka G, Uberle B, Vas V, Eckl C, Andrä I, Schiemann M, Oostendorp RA, Scharffetter-Kochanek K, Kestler HA, Zheng Y, Geiger H
Nature 2013 Nov 21;503(7476):392-6
Nature 2013 Nov 21;503(7476):392-6
NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway.
Li Y, Li B, Wan X, Zhang W, Zhong L, Tang SJ
Molecular brain 2012 Jan 4;5:1
Molecular brain 2012 Jan 4;5:1
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
Endo M, Doi R, Nishita M, Minami Y
Journal of cell science 2012 Apr 15;125(Pt 8):2017-29
Journal of cell science 2012 Apr 15;125(Pt 8):2017-29
WNT5A signaling contributes to Aβ-induced neuroinflammation and neurotoxicity.
Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
PloS one 2011;6(8):e22920
PloS one 2011;6(8):e22920
Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence.
Bitler BG, Nicodemus JP, Li H, Cai Q, Wu H, Hua X, Li T, Birrer MJ, Godwin AK, Cairns P, Zhang R
Cancer research 2011 Oct 1;71(19):6184-94
Cancer research 2011 Oct 1;71(19):6184-94
Evidence for altered Wnt signaling in psoriatic skin.
Gudjonsson JE, Johnston A, Stoll SW, Riblett MB, Xing X, Kochkodan JJ, Ding J, Nair RP, Aphale A, Voorhees JJ, Elder JT
The Journal of investigative dermatology 2010 Jul;130(7):1849-59
The Journal of investigative dermatology 2010 Jul;130(7):1849-59
Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons.
Bodmer D, Levine-Wilkinson S, Richmond A, Hirsh S, Kuruvilla R
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jun 10;29(23):7569-81
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jun 10;29(23):7569-81
Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10.
Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G
Arteriosclerosis, thrombosis, and vascular biology 2008 Mar;28(3):504-10
Arteriosclerosis, thrombosis, and vascular biology 2008 Mar;28(3):504-10
Wnt5a is expressed in murine and human atherosclerotic lesions.
Christman MA 2nd, Goetz DJ, Dickerson E, McCall KD, Lewis CJ, Benencia F, Silver MJ, Kohn LD, Malgor R
American journal of physiology. Heart and circulatory physiology 2008 Jun;294(6):H2864-70
American journal of physiology. Heart and circulatory physiology 2008 Jun;294(6):H2864-70
Dickkopf-1 is a master regulator of joint remodeling.
Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
Nature medicine 2007 Feb;13(2):156-63
Nature medicine 2007 Feb;13(2):156-63
Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E
Molecular and cellular neurosciences 2006 Feb;31(2):251-62
Molecular and cellular neurosciences 2006 Feb;31(2):251-62
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
Masckauchán TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
Molecular biology of the cell 2006 Dec;17(12):5163-72
Molecular biology of the cell 2006 Dec;17(12):5163-72
The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation.
Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N
Blood 2006 Aug 1;108(3):965-73
Blood 2006 Aug 1;108(3):965-73
Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo.
Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M
Proceedings of the National Academy of Sciences of the United States of America 2003 Mar 18;100(6):3422-7
Proceedings of the National Academy of Sciences of the United States of America 2003 Mar 18;100(6):3422-7
Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
Polesskaya A, Seale P, Rudnicki MA
Cell 2003 Jun 27;113(7):841-52
Cell 2003 Jun 27;113(7):841-52
Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
Polesskaya A, Seale P, Rudnicki MA
Cell 2003 Jun 27;113(7):841-52
Cell 2003 Jun 27;113(7):841-52
Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.
Ahumada A, Slusarski DC, Liu X, Moon RT, Malbon CC, Wang HY
Science (New York, N.Y.) 2002 Dec 6;298(5600):2006-10
Science (New York, N.Y.) 2002 Dec 6;298(5600):2006-10
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Wnt-5a by Western Blot. Western blot shows lysates of mouse brain and lactating mammary tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-5a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Wnt-5a in Mouse Embryonic Rib. Wnt-5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Wnt-5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
