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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-41244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT5A Recombinant Rabbit Monoclonal Antibody (JU30-30)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU30-30
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WNT5A in Hela cell lysate using WNT5A Recombinant Monoclonal Antibody (Product # MA5-41244) at a1:1,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of WNT5A in PC-3M cells (green) using WNT5A Recombinant Monoclonal Antibody (Product # MA5-41244). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of WNT5A in paraffin-embedded human lung cancer tissue using WNT5A Recombinant Monoclonal Antibody (Product # MA5-41244). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of WNT5A in SH-SY5Y cells. The cells were fixed, permeabilized and stained with WNT5A Recombinant Monoclonal Antibody (Product # MA5-41244) at a dilution of 1:50 (red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. Unlabelled control (cells without incubation with primary antibody; black).
