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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- EB06288 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB06288, RRID:AB_2188756
- Product name
- Goat anti-SIRT4 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-SIRT4 Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
SRCGELLPLIDPC- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06288 (0.05ug/ml) staining of Human Kidney (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 overexpressing SIRT4 (RC212226) and probed with EB05503 (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06288 (2µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
