TA321815

antibody from OriGene

Targeting: SIRT4 SIR2L4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321815

Provider

OriGene

Product name

Anti-SIRT4 Antibody

Antibody type

Polyclonal

Antigen

Fusion protein corresponding to a region derived from 45-314 amino acids of human sirtuin 4

Description

Anti-SIRT4 rabbit polyclonal antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.9mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 35kDa. Positive control: MCF7 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SIRT4 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)

Validation comment

IHC