Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6590 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6590, RRID:AB_1580645
- Product name
- SIRT4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SIRT4.
- Antigen sequence
SRCGELLPLIDPC
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.
Frye RA
Biochemical and biophysical research communications 1999 Jun 24;260(1):273-9
Biochemical and biophysical research communications 1999 Jun 24;260(1):273-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SIRT4 polyclonal antibody (Cat # PAB6590) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing SIRT4 and probed with SIRT4 polyclonal antibody (Cat # PAB6590) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SIRT4 polyclonal antibody (Cat # PAB6590) (2.5 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)