Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP16214PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP16214PU-N, RRID:AB_1928181
- Product name
- anti SIRT4 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Peptide from C-Terminus of the protein sequence according to NP_036372.1
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
Submitted references Reduced expression of SIRT1 is associated with diminished glucose-induced insulin secretion in islets from calorie-restricted rats.
Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase.
do Amaral ME, Ueno M, Oliveira CA, Borsonello NC, Vanzela EC, Ribeiro RA, Alves PL, Barbosa HC, Carneiro EM, Boschero AC
The Journal of nutritional biochemistry 2011 Jun;22(6):554-9
The Journal of nutritional biochemistry 2011 Jun;22(6):554-9
Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase.
Ahuja N, Schwer B, Carobbio S, Waltregny D, North BJ, Castronovo V, Maechler P, Verdin E
The Journal of biological chemistry 2007 Nov 16;282(46):33583-92
The Journal of biological chemistry 2007 Nov 16;282(46):33583-92
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 overexpressing SIRT4 and probed with AP16214PU-N SIRT4 antibody (mock transfection in first lane), tested by Origene.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16214PU-N SIRT4 antibody staining of Human Kidney lysate at 0.05 µg/ml (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16214PU-N SIRT4 antibody staining of Paraffin Embedded Human Skeletal Muscle at 2.5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16214PU-N SIRT4 antibody staining of Paraffin Embedded Human Pancreas at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.