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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX50032 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX50032, RRID:AB_11174641
- Product name
- DACT3 antibody [2A5]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50, GTX50032) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
