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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1071 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cathepsin C/DPPI Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cathepsin C/DPPI in direct ELISAs and Western blots. In direct ELISAs,approximately 20% cross-reactivity with recombinant mouse Cathepsin C and recombinant rat Cathepsin C is observed and less than 1% cross-reactivity with recombinant human (rh) Cathepsin H and rhCathepsin X/Z/P is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P53634- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype.
Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases.
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Promiscuous processing of human alphabeta-protryptases by cathepsins L, B, and C.
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Blommaert E, Péanne R, Cherepanova NA, Rymen D, Staels F, Jaeken J, Race V, Keldermans L, Souche E, Corveleyn A, Sparkes R, Bhattacharya K, Devalck C, Schrijvers R, Foulquier F, Gilmore R, Matthijs G
Proceedings of the National Academy of Sciences of the United States of America 2019 May 14;116(20):9865-9870
Proceedings of the National Academy of Sciences of the United States of America 2019 May 14;116(20):9865-9870
Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases.
Hamon Y, Legowska M, Hervé V, Dallet-Choisy S, Marchand-Adam S, Vanderlynden L, Demonte M, Williams R, Scott CJ, Si-Tahar M, Heuzé-Vourc'h N, Lalmanach G, Jenne DE, Lesner A, Gauthier F, Korkmaz B
The Journal of biological chemistry 2016 Apr 15;291(16):8486-99
The Journal of biological chemistry 2016 Apr 15;291(16):8486-99
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Cherepanova NA, Shrimal S, Gilmore R
The Journal of cell biology 2014 Aug 18;206(4):525-39
The Journal of cell biology 2014 Aug 18;206(4):525-39
Promiscuous processing of human alphabeta-protryptases by cathepsins L, B, and C.
Le QT, Min HK, Xia HZ, Fukuoka Y, Katunuma N, Schwartz LB
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7136-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7136-43
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, van den Berg A
Blood 2008 Feb 15;111(4):2339-46
Blood 2008 Feb 15;111(4):2339-46
Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
Genes & development 2006 Mar 1;20(5):543-56
Genes & development 2006 Mar 1;20(5):543-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cathepsin C/DPPI by Simple Western<SUP abp="262">TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 56 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cathepsin C/DPPI by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cathepsin C/DPPI in Human Lung Cancer Tissue. Cathepsin C/DPPI was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
