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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA303752 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA303752, RRID:AB_2246075
- Product name
- Rabbit monoclonal antibody against DLC8 (N-term) (EP1660Y )
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against DLC8 (N-term) (EP1660Y )
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DYNLL1
- Isotype
- IgG
- Antibody clone number
- EP1660Y
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - DLC8 antibody [EP1660Y]; Anti-DYNLL1 antibody [EP1660Y] at 1/10000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 10 kDa.Observed band size : 10 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - DLC8 antibody [EP1660Y]; TA303752 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% milk for 1 hour at room temperature. The sample was incubated with primary antibody (1/400; 1% milk in PBS) for 16 hours at 4?°C. An Alexa Fluor?488-conjugated Goat polyclonal to rabbit IgG (1/1000) was used as secondary antibody.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA303752 used in IP.SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence coverage from each IP. (B) Silver-stained gel showing the purification of FLAG-SKAP from chicken DT40 cells relative to controls. The indicated proteins were identified by excising them from a gel and analyzing them by mass spectrometry.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - DLC8 antibody [EP1660Y]; Immunohistochemical staining of paraffin embedded human liver using TA303752 (1/100).
- Validation comment
- IHC
