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- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 14447-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14447-1-AP, RRID:AB_2292527
- Product name
- CYP17A1 antibody
- Antibody type
- Polyclonal
- Description
- CYP17A1 antibody (Cat. #14447-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A Novel SEMA3G Mutation in Two Siblings Affected by Syndromic GnRH Deficiency.
Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary.
Effect of the spatial-temporal specific theca cell Cyp17 overexpression on the reproductive phenotype of the novel TC17 mouse.
Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells.
WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.
Expression and localisation of ephrin-B1, EphB2, and EphB4 in the mouse testis during postnatal development.
3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.
Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance, mitochondrial dysfunction and oxidative stress.
Features of the fetal gonad in androgen synthesis in the postpubertal testis are preserved in complete androgen insensitivity syndrome due to a novel genetic splice site donor variant in androgen receptor gene intron 1.
The Involvement of Granulosa Cells in the Regulation by Gonadotropins of Cyp17a1 in Theca Cells.
Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.
Deleting the mouse Hsd17b1 gene results in a hypomorphic Naglu allele and a phenotype mimicking a lysosomal storage disease.
Transcriptome Analysis of WHV/c-myc Transgenic Mice Implicates Cytochrome P450 Enzyme 17A1 as a Promising Biomarker for Hepatocellular Carcinoma.
Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.
Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells.
Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.
Increased expression of CYP17A1 indicates an effective targeting of the androgen receptor axis in castration resistant prostate cancer (CRPC).
Intra-tissue steroid profiling indicates differential progesterone and testosterone metabolism in the endometrium and endometriosis lesions.
Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.
Oleari R, André V, Lettieri A, Tahir S, Roth L, Paganoni A, Eberini I, Parravicini C, Scagliotti V, Cotellessa L, Bedogni F, De Martini LB, Corridori MV, Gulli S, Augustin HG, Gaston-Massuet C, Hussain K, Cariboni A
Neuroendocrinology 2021;111(5):421-441
Neuroendocrinology 2021;111(5):421-441
Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary.
Alam J, Ogawa K
Reproductive biology 2021 Sep;21(3):100511
Reproductive biology 2021 Sep;21(3):100511
Effect of the spatial-temporal specific theca cell Cyp17 overexpression on the reproductive phenotype of the novel TC17 mouse.
Secchi C, Belli M, Harrison TNH, Swift J, Ko C, Duleba AJ, Stupack D, Chang RJ, Shimasaki S
Journal of translational medicine 2021 Oct 15;19(1):428
Journal of translational medicine 2021 Oct 15;19(1):428
Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells.
Su Y, Tian Z, Qi X, Luo D, Liu L, Liu S, Zheng D, Wei F, He Z, Guan Q
Nutrition & metabolism 2021 May 26;18(1):53
Nutrition & metabolism 2021 May 26;18(1):53
WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.
Habara O, Logan CY, Kanai-Azuma M, Nusse R, Takase HM
Development (Cambridge, England) 2021 May 1;148(9)
Development (Cambridge, England) 2021 May 1;148(9)
Expression and localisation of ephrin-B1, EphB2, and EphB4 in the mouse testis during postnatal development.
Gofur MR, Alam J, Ogawa K
Reproductive biology 2020 Sep;20(3):321-332
Reproductive biology 2020 Sep;20(3):321-332
3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.
Lu J, Lu J, Chen Y, Feng Z, Liu S, Guan S
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Oct;144:111575
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Oct;144:111575
Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance, mitochondrial dysfunction and oxidative stress.
Peng Y, Yang X, Luo X, Liu C, Cao X, Wang H, Guo L
Biological research 2020 Oct 27;53(1):50
Biological research 2020 Oct 27;53(1):50
Features of the fetal gonad in androgen synthesis in the postpubertal testis are preserved in complete androgen insensitivity syndrome due to a novel genetic splice site donor variant in androgen receptor gene intron 1.
Jarzabek K, Koda M, Chrusciel M, Kanczuga-Koda L, Sobczynska-Tomaszewska A, Rahman NA, Wolczynski S
The Journal of steroid biochemistry and molecular biology 2019 Oct;193:105420
The Journal of steroid biochemistry and molecular biology 2019 Oct;193:105420
The Involvement of Granulosa Cells in the Regulation by Gonadotropins of Cyp17a1 in Theca Cells.
Kakuta H, Iguchi T, Sato T
In vivo (Athens, Greece) 2018 Nov-Dec;32(6):1387-1401
In vivo (Athens, Greece) 2018 Nov-Dec;32(6):1387-1401
Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.
Tian H, Chen S, Leng Y, Li T, Li Z, Chen H, Zhang Q
Environmental toxicology 2018 Mar;33(3):351-360
Environmental toxicology 2018 Mar;33(3):351-360
Deleting the mouse Hsd17b1 gene results in a hypomorphic Naglu allele and a phenotype mimicking a lysosomal storage disease.
Jokela H, Hakkarainen J, Kätkänaho L, Pakarinen P, Ruohonen ST, Tena-Sempere M, Zhang FP, Poutanen M
Scientific reports 2017 Nov 27;7(1):16406
Scientific reports 2017 Nov 27;7(1):16406
Transcriptome Analysis of WHV/c-myc Transgenic Mice Implicates Cytochrome P450 Enzyme 17A1 as a Promising Biomarker for Hepatocellular Carcinoma.
Wang F, Huang J, Zhu Z, Ma X, Cao L, Zhang Y, Chen W, Dong Y
Cancer prevention research (Philadelphia, Pa.) 2016 Sep;9(9):739-49
Cancer prevention research (Philadelphia, Pa.) 2016 Sep;9(9):739-49
Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.
Hakkarainen J, Jokela H, Pakarinen P, Heikelä H, Kätkänaho L, Vandenput L, Ohlsson C, Zhang FP, Poutanen M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Sep;29(9):3806-16
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Sep;29(9):3806-16
Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells.
Robitaille CN, Rivest P, Sanderson JT
Toxicological sciences : an official journal of the Society of Toxicology 2015 Jan;143(1):126-35
Toxicological sciences : an official journal of the Society of Toxicology 2015 Jan;143(1):126-35
Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.
Tong A, Jia A, Yan S, Zhang Y, Xie Y, Liu G
International journal of clinical and experimental pathology 2014;7(7):4415-21
International journal of clinical and experimental pathology 2014;7(7):4415-21
Increased expression of CYP17A1 indicates an effective targeting of the androgen receptor axis in castration resistant prostate cancer (CRPC).
Bremmer F, Jarry H, Strauß A, Behnes CL, Trojan L, Thelen P
SpringerPlus 2014;3:574
SpringerPlus 2014;3:574
Intra-tissue steroid profiling indicates differential progesterone and testosterone metabolism in the endometrium and endometriosis lesions.
Huhtinen K, Saloniemi-Heinonen T, Keski-Rahkonen P, Desai R, Laajala D, Ståhle M, Häkkinen MR, Awosanya M, Suvitie P, Kujari H, Aittokallio T, Handelsman DJ, Auriola S, Perheentupa A, Poutanen M
The Journal of clinical endocrinology and metabolism 2014 Nov;99(11):E2188-97
The Journal of clinical endocrinology and metabolism 2014 Nov;99(11):E2188-97
Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.
Chrusciel M, Vuorenoja S, Mohanty B, Rivero-Müller A, Li X, Toppari J, Huhtaniemi I, Rahman NA
Journal of cell science 2013 Apr 15;126(Pt 8):1845-57
Journal of cell science 2013 Apr 15;126(Pt 8):1845-57
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14447-1-AP(CYP17A1 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CYP17A1 antibody 14447-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 14447-1-AP(CYP17A1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 14447-1-AP(CYP17A1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
