MA5-34697

antibody from Invitrogen Antibodies

Targeting: CYP17A1 CPT7, CYP17, P450C17, S17AH

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34697

Provider

Invitrogen Antibodies

Product name

CYP17A1 Recombinant Rabbit Monoclonal Antibody (JB93-32)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: HepG2, Hela, SH-SY-5Y, human breast tissue, human kidney tissue, mouse kidney tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB93-32

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytochrome P450 17A1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytochrome P450 17A1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytochrome P450 17A1 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 17A1 in paraffin-embedded mouse kidney tissue. Samples were incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 17A1 in paraffin-embedded human kidney tissue. Samples were incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 17A1 in paraffin-embedded human breast tissue. Samples were incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Cytochrome P450 17A1 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Cytochrome P450 17A1 monoclonal antibody (Product # MA5-34697) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.